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Pentrtm/d-topotm 克隆试剂盒

WebRNAi vector had been successfully constructed using GATEWAYTM cloning technology with the 3'UTRMmpma was used as double-stranded RNA (dsRNA) trigger sequence, pENTRTM/D-TOPO®as entry vector, and pANDA plasmid as destination vector. Web17. sep 2015 · Store Box -20C.Item Concentration Amount pENTR vector,TOPO -adapted (pENTR 15-20ng/l linearized plasmid DNA 50%glycerol 50 mM Tris-HCl, pH 7.4 mMEDTA mMDTT 0.1% Triton X-100 100 g/ml BSA 30 bromophenolblue 20 dNTPMix 12.5 mM dATP 12.5 mM dCTP 12.5 mM dGTP 12.5 mM dTTP water,pH SaltSolution 1.2 NaCl0.06 mlM13 …

Addgene: Vector Database - pENTR/H1/TO

WebVector database is a digital collection of vector backbones assembled from publications and commercially available sources. This is a free resource for the scientific community that is compiled by Addgene. This page is informational only - this vector is NOT available from Addgene - please contact the manufacturer for further details. WebThe pENTR/D-TOPO Cloning Kits utilize a highly efficient, 5-minute cloning strategy (TOPO Cloning) to directionally clone a blunt-end PCR product into a vector for entry into the … cost for weed control https://southernkentuckyproperties.com

HTP -pENTR-D-TOPO定向克隆载体 - 豆丁网

Webthermo fisher pentrtm sd d topotm vectors Pentrtm Sd D Topotm Vectors, supplied by thermo fisher, used in various techniques. Bioz Stars score: 96/100, based on 7 PubMed … http://www.haoranbio.com/ProductDetails-601088.aspx WebpENTR™⁄D-TOPO™ Cloning Kit contains pENTR⁄D-TOPO™ vector, dNTPs, salt solution, sterile water, universal M13 sequencing primers, OneShot™ TOP10 Chemically E. coli, … Simple Access to the Gateway™ System For access to the Gateway™ System, just … TaqMan Real-Time PCR Assays. Antibodies. Oligos, Primers & Probes cost for web designer

Champion™ pET151 Directional TOPO™ Expression Kit with BL21 …

Category:AtRGS1-GFP 融合蛋白对 AtRGS1 细胞定位的研究

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Pentrtm/d-topotm 克隆试剂盒

Pentrtm 5 Topo Vector Thermo Fisher Bioz

Web本试剂盒是利用拓扑异构酶(Topoisomerase)高效快速连接DNA片段的原理进一步研发而成，与传统的T4连接酶相比，具有以下优势：1）快速，仅需 1-5 min即可完成连接反应。 2）高效，无自连，阳性克隆率接近100%，无需设置蓝白斑筛选；3）操作简单，从连接到涂板仅需 15-20 min。 操作过程省去冰浴、热激和1 h复苏。 4）可连接长达5 kb目的产 … Web5. jan 2014 · 唐雯，等.人组织因子基因RNAi 载体pENTR TM /U6-TF-shRNA 的构建与鉴定 ISSN 1673-8225 CN 21-1539/R CODEN: ZLKHAH 259 www.zglckf.com 中山大学附属第一医院 儿科, 广东省广 南方医科大学南方医院检验医学 中心，广东省广州 方医科大学中医药学院中医症候 实验室，广东省广 庆市人 ...

Pentrtm/d-topotm 克隆试剂盒

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Web51TRF)观察GFP表达情况; 7 d后, 收集并提取重 组农杆菌转化系(含AtRGS1-GFP融合基因)、对 照(只含GFP基因)和空白对照(正常悬浮细胞系)的 细胞总蛋白。10% SDS-PAGE电泳分离, 然后转 膜(NC, Amersham公司)、漂洗、封闭, 一抗GFP 抗体(Santa cruz公司)孵育、洗膜、显色。 WebpENTR/D-TOPO 克隆试剂盒利用高效的 5 分钟克隆策略（TOPO 克隆）将平末端 PCR 产物定向克隆成为进入 Gateway 系统或 MultiSite Gateway 系统的载体。 平末端 PCR 产物以高 …

WebThe amplified PCR product was inserted into a TOPO donor vector using the pENTRTM/D-TOPO Cloning Kit (Invitrogen), and then inserted into the destination vector, pKGWFS7, using the Gateway LR Clonase™ II enzyme mix (Invitrogen). 5 … Web概述. 本试剂盒是一种高效、便利的 PCR 产物克隆专用试剂盒。. 以 Taq DNA 聚合酶扩增的 PCR 条带有单个碱基A末端，利用 T4 DNA Ligase 将其与 pUCm-T 载体连接形成克隆质粒。. 可以通过蓝白斑筛选有插入片段的重组克隆。.

Web丁香通-专业的科研采购信息和实验资讯平台 WebThe Directional TOPO™ Expression box contains 200 ng of linearized, topoisomerase I-activated Champion™ pET vector; sterile water; dNTPs; 10X PCR Buffer; salt solution; control template and primers; primers for sequencing or PCR screening; and an expression control. Store at -20°C.

Webpct这个词汇来自于类固醇循环中，指的是类固醇使用之后的恢复阶段。 但你要注意的是： 1、pct不只是简单的“恢复睾酮”！ 2、在类固醇循环后，pct还应该包括类似于肝脏恢复、 …

Web本试剂盒是利用拓扑异构酶（Topoisomerase）高效快速连接DNA片段的原理进一步研发而成，与传统的T4连接酶相比，具有以下优势：1）快速，仅需1-5分钟即可完成连接反应。 2）高效，无自连现象，阳性克隆率接近100%，无需设置蓝白斑筛选；3）操作简单，从连接到涂板仅需15-20 min。 操作过程省去冰浴、热激和1 h复苏。 4）可连接长达5 kb目的产 … cost for web page designWeb6. nov 2014 · Store Box -20C.Item Concentration Amount pENTR vector,TOPO -adapted (pENTR 15-20ng/l linearized plasmid DNA 50%glycerol 50 mM Tris-HCl, pH 7.4 mMEDTA … cost for water lineWebThermo Fisher pentrtm sd d topotm vector Pentrtm Sd D Topotm Vector, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed … breakfast places near atlantic stationWebGateway pENTR-D-TOPO vector是效率很高的一个entry vector试剂盒,适合转基因的克隆,在细菌中表达 Vector Map: pENTR/D-TOPO NAME: pENTR/D-TOPO RESISTANT MARKER: … cost for wedding photographersWeb11. nov 2013 · ZAR1 transgene effects in maize plants. (a) Inbred transgenic plants over-expressing ZAR1 (Pro ZmUBI:ZAR1) grown in the field: plants and immature ears during the growing season are shown.(b) Dry mass of whole plant, stalk, primary ear and leaf area at the V16 stage of hybrid plants over-expressing ZAR1.Black bars: transgenic; red bars: … breakfast places near apex ncWebpENTR/D-TOPO 克隆试剂盒利用高效的 5 分钟克隆策略（TOPO 克隆）将平末端 PCR 产物定向克隆成为进入 Gateway 系统或 MultiSite Gateway 系统的载体。 平末端 PCR 产物以高 … breakfast places near andover maWebpENTR D-TOPO. Directional TOPO® cloning vector, for creating a Gateway® entry vector that is suitable for recombination with a destination vector. Sequence Author: Thermo Fisher … breakfast places near asu