Nphs2 cre

Author: fbam

August undefined, 2024

Web1 aug. 2024 · Cre 工具鼠的种类及应用 Cre 工具鼠主要分为 3 类：全身性 Cre、组织特异性 Cre 和配体或药物诱导 Cre。全身性 Cre：通过诱导剂来调节驱动 Cre 重组酶的启动子活 … WebNPHS2 pathogenic variants are the most common podocyte gene defects. Yet, pathogenic variants are found only in a minority of SRNS patients. No homozygotes or compound …

Supplementary Table S1. Differentially-expressed genes in …

WebThe p2.5P-Cre transgene was designed with a 2.5 kb fragment of the human podocin (or NPHS2) promoter/enhancer region upstream of a Cre recombinase cassette followed by a murine protamine poly-adenylation signal. The donating investigator reports that the transgene was microinjected into (C57BL/6 X SJL)F2 mouse eggs. Web7 sep. 2024 · The CD16/7-nephrin activation model allows for precisely-timed induction of nephrin tyrosine phosphorylation in live cells by addition of specific antibodies that cluster the extracellular CD16 domain and results in intracellular tyrosine phosphorylation of nephrin’s cytoplasmic tail. can you mix different oil weights

Partial podocyte replenishment in experimental FSGS derives …

WebNphs2-CreERT2 品系全名 C57BL/6JSmoc- Nphs2tm1 (IRES-CreERT2)Smoc 目录号 NM-KI-00128 品系状态胚胎冻存加入购物车导出PDF 基因信息基因名 Nphs2 基因曾用名 … WebB6.Cg-Tg(NPHS2-cre)295Lbh/J. Stock No: 008205. Protocol 20018: Standard PCR Assay - Tg(NPHS2-cre)295Lbh. Version 1. 0. Notes. ... NPHS2: oIMR7338: CTA GGC CAC AGA ATT GAA AGA TCT : Internal Positive Control Forward: A: oIMR7339: GTA GGT GGA AAT TCT AGC ATC ATC C: Internal Positive Control Reverse: A: http://geneandpeace.com/gongjuxiaoshu/202411/415.html briley pumpkin patch greenville nc

Protocol 20018 - Tg(NPHS2-cre)295Lbh - jax.org

Web5 apr. 2014 · 肾小管组织特异性cre工具鼠目前已应用于肾小管基因敲除的cre工具鼠主要由以下启动子驱动，其中在近端小管中特异性较高的启动子为GGT，SGLT2，PEPCKKAP， … WebThe NPHS2 gene provides instructions for making a protein called podocin. Podocin is primarily found in the kidneys, which are organs that filter waste products from the blood … briley rasmussenWeb019382 FVB.Cg- Myh9 tm1.1Gac Tg(NPHS2-cre)295Lbh/Mmjax When Myh9 flox mice are crossed with Pod::Cre mice resulting offspring will have exon 1 deleted in kidney podocytes. Double mutant mice on the C57BL/6 background do not develop spontaneous proteinuria or renal insufficiency, however, when subjected to environmental stress via doxyrubicin … can you mix different speed rated tyres

"Web24 jul. 2012 · The Nphs2-Cre mouse line (3) expresses Cre-recombinase tran-script under control of a 2.5-kb mouse podocyte-speciﬁc podocin promoter. The Arnt ﬂox/ﬂox mice were kindly provided by Dr. Frank J. Gonzalez (22). To generate mice with podocyte-selective deletion of Arnt, Nphs2-Cre mice were bred to Arnt ﬂox/ﬂox homozygotes. " - Nphs2 cre

Nphs2 cre

Web28 okt. 2024 · The epidermal growth factor receptor (EGFR) is expressed in numerous cell types in the adult mammalian kidney and is activated by a family of EGF-like ligands. EGFR activation has been implicated in a variety of physiologic and pathophysiologic functions. There is increasing evidence that aberrant EGFR activation is a mediator of progressive … Web15 aug. 2024 · Rosa26-CreERT2 小鼠种系能够通过体内他莫昔芬诱导对floxed基因表达进行时间控制。. 也就是在Rosa26位点插入了CreERT2基因使其能能够稳定表达，当进行他 …

Did you know?

Web19 nov. 2002 · Twenty- four transgenic founders were generated in which Cre recombinase was placed under the regulation of a 2.5-kb fragment of the human NPHS2 promoter. … Web15 okt. 2024 · NPHS2-cre mice were purchased from Jackson Laboratory (Bar Harbor, ME, USA). Podocyte-specific Crb2 knockout mice were generated by breeding Crb2 fl/fl mice …

WebPodocin-Cre mice (designated Nphs2Cre) were obtained from Jackson (008205, B6. Cg-Tg (NPHS2-cre) 295Lbh/J). The Lonp1floxed mice (designated Lonp1fl/fl) were generous … WebFirst, Nphs2 encodes podocin, an essential component of the slit diaphragm, a cell-cell junctional structure between adjacent podocytes, which is one of the most important …

Web10 jan. 2024 · scRNA-seq of BTBR ob/ob mouse kidneys with early DN. We generated BTBR ob/ob podocyte-reporter mice (BTBR.Cg-Lep ob/ob WiscJ;Gt (ROSA)26Sor tm4(ACTB−tdTomato,−EGFP)Luo;Tg(NPHS2-cre) 295Lbh; hereafter referred to as BTBR ob/ob or DN mice) to investigate the cellular and molecular changes that occur in DN. By … WebTg (NPHS2-cre/ERT2)#Mkas. Mutation details : A 2.5 kb human podocin (NPHS2) promoter (from BAC clone RP11-231PS) was used to drive expression of cre recombinase fused …

WebPartial Sequences from Addgene (3) Addgene has sequenced portions of this plasmid for verification. The results are shown below. Leading primers are indicated on the first line …

Web11 feb. 2010 · Cre-mediated recombination. To determine the ability of Cre recombinase on tamoxifen-inducible selective recombination of loxP sites, we crossbred NPHS2–CreER … briley rd spring city tnWeb7 jun. 2016 · Podocyte confetti reporter mice. To study the potential ability of podocytes to regenerate following the induction of experimental FSGS, adult (8–10 wk old) NPHS2 Cre /Confetti TG/WT (podocin-cre/confetti) reporter mice, generously provided by Dr. Katalin Susztak (University of Pennsylvania, Philadelphia, PA) and described by Tao et al. (), … can you mix different types of oilWebCre 蛋白是一种重组酶，是causes recombination的缩写，于1981 年从 P1 噬菌体中被发现，属于 λInt 酶超基因家族。 Cre 重组酶基因编码区序列全长1029bp，编码 38kDa 蛋白 … briley raptor 10/22 barrelWeb20 mrt. 2024 · NPHS2 Cre PAR-1 Active+/− mice were also in renal failure as evidenced by their significantly higher levels of serum creatinine and urea compared with littermate controls (Figure 4 c and d, respectively). Furthermore, NPHS2 Cre PAR-1 Active+/− mice died between the ages of 39 and 45 days (Figure 4 e). can you mix dilantin with d5nsWeb7 aug. 2024 · 2.1 新的Cre品系需要做的四件事：. 1) 了解小鼠来源，背景信息及发表的文章；. 2) 建立基因型鉴定方法，确认小鼠正确；. 3) 验证Cre在目标组织中的表达. 4) 验 … can you mix different watt solar panelsWebnphs2(Cre Ert2) nphs2(Cre) 备注：除以上列出的Cre大鼠模型外，如您需要其他品系的大鼠模型，可拨打400-680-8038联系我们，以获取专属的定制服务报价。 can you mix different weights of motor oilWeb21 feb. 2024 · Tg (NPHS2-cre)295Lbh. Mutation details : The human NPHS2 promoter, including the entire 5' untranslated region, was used to drive cre recombinase … briley raptor barrel