WebOct 28, 2014 · Glycan SPE C18 and Graphitized Carbon Protocols (P0710) To remove peptides, protein, and other contaminants present in a glycan sample, use a C18 … WebSPE Cartridge, Sample Preparation Chromatography - Hawach Hawach SPE cartridges consist of medical PP empty tube, medical PE sieve plate, and a fixed adsorbent, used in …
Glycan SPE C18 and Graphitized Carbon Protocols (P0710)
WebiSPE-HILIC cartridges are designed for the purification of polar and hydrophilic compounds using Hydrophilic Interaction Liquid Chromatography (HILIC). The packed stationary phases are charge modulated … WebApr 18, 2024 · Glycans of interest are physically retained on the SPE cartridge through strong electrostatic interactions between PCG and the APTS label. In order to effectively release APTS-labeled glycans from the PCG SPE cartridges, eluents containing millimolar (mM) concentrations of a strong, volatile ion-pairing reagent, such as trifluoroacetic acid ... crypto hari ini
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WebFigure 1.Schematic for preparing 2-AB labeled glycans from a glycoprotein using GlycoWorks. Consumables included as part of the GlycoWorks solution are highlighted in blue. ... use of different SPE device formats (single-use cartridges and high-throughput plates), and that the included human IgG control standard can be used for method ... Webphase extraction, 2. graphitized carbon solid-phase extraction, 3. ultrafiltration, 4. ethanol precipitation, and 5. no purification. The procedures for each method of purification are shown in Tables 1 to 5. Table 1 Purification by Reversed Phase Solid-Phase Extraction Step Procedure 1 Prepare an Oasis£ HLB 1 cc cartridge or a SupelTM-Select ... The Porous Graphitic Carbon (PGC) Solid Phase Extraction (SPE) procedure is applicable to released glycans and glycopeptides. Trifluoroacetic acid (TFA) should be used. Due to high back pressure during the 30% acetic acid conditioning step, this procedure will not work well with the vacuum manifold. Do not … See more Do not allow air to enter the cartridge bed! 1. Pass 1ml 1M NaOH through cartridge bed to waste. 2. Pass 2ml MilliQ water through cartridge bed to waste. 3. Pass 1ml 30% acetic acid through cartridge bed to waste. 4. Pass 2ml … See more Do not let air enter the cartridge bed. Pass 1ml Wash Solvent through bed and collect effluent in same tube as above. See more cryptoguards token